<b>Microrna 138 Upregulation is Associated with Decreasing Levels of <i>CCND1</i> Gene Expression and Promoting Cell Death in Human Prostate Cancer Cell Lines</b>

Background: This research intended to discover the significance of miR-138 (microRNA 138) on expression profile, proliferation, and associated regulatory mechanisms in prostate cancer (PCa). Methods: Thirty-five specimens were studied evaluate level miR138 by RT-qPCR (Quantitative reverse transcription polymerase chain reaction). Bioinformatics analysis was performed search for target genes miR-138; ABL1 (ABL proto-oncogene 1, non-receptor tyrosine kinase), CCND1 (cyclin D1), CCND3 D3), VIM (vimentin), TWIST1 (twist family bHLH factor 1), HIF1A (hypoxia-inducible 1 subunit alpha), TERT (telomerase transcriptase) selected. Then, biological role progression PCa investigated using luciferase reporter gene assay. Finally, overexpression proliferation cell lines analyzed MTT (3-(4, 5-dimethylthiazol-2-Yl)-2, 5-diphenyltetrazolium bromide, Sigma, Germany) Results: showed that downregulated tissues lines. assay demonstrated negatively correlated with PC3 line. Moreover, predicted be line based results Substantially, over-expression miR138-5p mimic could inhibit Lastly, inhibited proliferative capacities DU-145 cells. Conclusion: Our introduces as a negative regulator an inhibitory impact rate mechanism utilized design selection remedial miRNA-based approaches.